The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division1-3. CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. Thus, when a CFSE-labeled cell divides, its progeny are endowed with half the number of carboxyfluorescein-tagged molecules and thus each cell division can be assessed by measuring the corresponding decrease in cell fluorescence via Flow cytometry. The capacity of CFSE to label lymphocyte populations with a high fluorescent intensity of exceptionally low variance, coupled with its low cell toxicity, make it an ideal dye to measure cell division. Since it is a fluorescein-based dye it is also compatible with a broad range of other fluorochromes making it applicable to multi-color flow cytometry. This article describes the procedures typically used for labeling mouse lymphocytes for the purpose of monitoring up to 8 cell divisions. These labeled cells can be used both for in vitro and in vivo studies

Shuttle-launch triangular space station

A triangular space station deployable in orbit is described. The framework is comprized of three trusses, formed of a pair of generally planar faces consistine of foldable struts. The struts expand and lock into rigid structural engagement forming a repetition of equilater triangles and nonfolding diagonal struts interconnecting the two faces. The struts are joined together by node fittings. The framework can be packaged into a size and configuration transportable by a space shuttle. When deployed, the framework provides a large work/construction area and ample planar surface area for solar panels and thermal radiators. A plurity of modules are secured to the framework and then joined by tunnels to make an interconnected modular display. Thruster units for the space station orientation and altitude maintenance are provided

Monopropellant combustion system

An apparatus and method are provided for decomposition of a propellant. The propellant includes an ionic salt and an additional fuel. Means are provided for decomposing a major portion of the ionic salt. Means are provided for combusting the additional fuel and decomposition products of the ionic salt

Bystander B cells rapidly acquire antigen receptors from activated B cells by membrane transfer: a novel mechanism for enhancing specific antigen presentation

The B cell antigen receptor (BCR) efficiently facilitates the capture and processing of a specific antigen for presentation on MHC class II molecules to antigen specific CD4+ T cells (1). Despite this, the majority of B cells are only thought to play a limited role in CD4+ T cell activation since BCRs are clonotypically expressed. Here we show, however, that activated B cells can, both in vitro and in vivo, rapidly donate their BCR to bystander B cells, a process that is mediated by direct membrane transfer between adjacent B cells and is amplified by the interaction of the BCR with specific antigen. This results in a dramatic expansion in the number of antigen-binding B cells in vivo, with the transferred BCR endowing recipient B cells with the ability to present specific antigen to antigen-specific CD4+ T cells

Airborne Measurements of Coastal Jet Transition around Point Conception, California

This is the publisher's version, also available electronically from http://journals.ametsoc.org/doi/abs/10.1175/MWR-D-13-00030.1Low-level winds along the Californian coast during spring and early summer are typically strong and contained within the cool, well-mixed marine boundary layer (MBL). A temperature inversion separates the MBL from the warmer free troposphere. This setup is often represented by a two-layer shallow-water system with a lateral boundary. Near a prominent point such as Point Conception, California, the fast-moving MBL flow is supercritical and can exhibit distinct features including a compression bulge and an expansion fan. Measurements from the University of Wyoming King Air research aircraft on 19 May 2012 during the Precision Atmospheric MBL Experiment (PreAMBLE) captured wind in excess of 14 m s−1 off of Point Conception under clear skies and wind ~2 m s−1 east of San Miguel in the California Bight. A compression bulge was identified upwind of Point Conception. When the flow rounds the point, the MBL undergoes a near collapse and there is a spike in MBL height embedded in the general decrease of MBL height with greater turbulence just downwind that is associated with greater mixing through the inversion layer. Lidar and in situ measurements reveal that transport of continental aerosol is present near the pronounced MBL height change and that there is a complex vertical structure within the Santa Barbara Channel. Horizontal pressure gradients are obtained by measuring the slope of an isobaric surface. Observations of wind and pressure perturbations are able to be linked through a simple Bernoulli relationship. Variation of MBL depth explains most, but not all of the variation of the isobaric surface

Airborne Observations of a Catalina Eddy

This is the publisher's version, also available electronically from http://journals.ametsoc.org/doi/abs/10.1175/MWR-D-13-00029.1Summertime low-level winds over the ocean adjacent to the California coast are typically from the north, roughly parallel to the coastline. Past Point Conception the flow often turns eastward, thereby generating cyclonic vorticity in the California Bight. Clouds are frequently present when the cyclonic motion is well developed and at such times the circulation is referred to as a Catalina eddy. Onshore flow south of the California Bight associated with the eddy circulation can result in a thickening of the low-level marine stratus adjacent to the coast. During nighttime hours the marine stratus typically expands over a larger area and moves northward along the coast with the cyclonic circulation. A Catalina eddy was captured during the Precision Atmospheric Marine Boundary Layer Experiment in June of 2012. Measurements were made of the cloud structure in the marine layer and the horizontal pressure field associated with the cyclonic circulation using the University of Wyoming King Air research aircraft. Airborne measurements show that the coastal mountains to the south of Los Angeles block the flow, resulting in enhanced marine stratus heights and a local pressure maximum near the coast. The horizontal pressure field also supports a south–north movement of marine stratus. Little evidence of leeside troughing south of Santa Barbara, California, was observed for this case, implying that the horizontal pressure field is forced primarily through topographic blocking by the coastal terrain south of Los Angeles, California, and the ambient large-scale circulation associated with the mean flow

Synthesis of Observations from the Precision Atmosphere Marine Boundary Layer Experiment (PreAMBLE)

Research flights during the Precision Atmospheric Marine Boundary Layer Experiment (PreAMBLE) in Southern California during May–June 2012 focused on three main features found in the nearshore marine boundary layer (MBL): the coastal jet (10 flights), the Catalina eddy (3 flights), and the initiation of a southerly surge (1 flight). Several topics were examined with case studies, but results from individual events may not represent typical conditions. Although these flights do not constitute a long-term set of data, observations from PreAMBLE are used to find common features. Two main topics are addressed: the MBL collapse into the expansion fan, and the subsequent transition into the Santa Barbara Channel (SBC). The midmorning to late afternoon flights occur during moderate to strong northerly wind. Slope of the MBL in the expansion fan varies and wave perturbations can be embedded within the expansion fan. As the cool MBL flow turns into the SBC, it moves underneath a deeper and warmer MBL that originates from the southeast over the warmer ocean. The temperature inversion between the cool and warm MBL erodes toward the east until there is only the inversion between the warm MBL and free troposphere. The dissipation of the lower layer into the SBC observed by the aircraft differs from previous conceptual models that depict a continuous MBL that thins and then deepens again in the SBC, which was inferred from sparse observations and numerical simulations. Only one flight within the SBC detected a hydraulic jump from 100 to 200 m above the surface